Fig. 3: Stromal MEN1 knockdown promoted the proliferation of endometrial epithelial cells. | Communications Biology

Fig. 3: Stromal MEN1 knockdown promoted the proliferation of endometrial epithelial cells.

From: Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI

Fig. 3

a Gene Ontology analysis of the differential upregulated genes as determined by RNA-seq. b Heatmap of differentially expressed fibroblast growth factors (FGFs) following MEN1 knockdown in decidualized hESCs (n = 3). c qRT-PCR analysis of FGFs in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 0 and 4 days (n = 3). d Schematic representation of the culture of assembloids. e Immunostaining of Ki67 and GFP in assembloids from the EEO+shCtrl and EEO+shMEN1 groups (n = 3); scale bar = 200 μm. f qRT-PCR analysis of PAEP and HSD17β2 in assembloids from the EEO+shCtrl and EEO+shMEN1 groups before and after treatment with E2, MPA, and 8Br-cAMP (n = 3). g Representative IHC staining images of Ki67 and p-ERK1/2 expression in the mid secretory phase human endometrium from the control and RIF groups (n = 3); scale bar = 200 μm. GE glandular epithelium, S stroma. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA.

Back to article page