Fig. 4: MEN1 depletion overactivated the WNT/β-catenin signaling pathway. | Communications Biology

Fig. 4: MEN1 depletion overactivated the WNT/β-catenin signaling pathway.

From: Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI

Fig. 4

a Gene Ontology analysis of the differential downregulated genes as determined by RNA-seq. b Gene set enrichment analysis (GSEA) plot of negative regulation of the WNT signaling pathway in the shMEN1 group as compared to that in the shCtrl group. c IF assay of β-catenin expression in stroma of MEN1f/f and MEN1d/d mice on D8; scale bar = 200 μm. d IF analysis of β-catenin expression in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3); scale bar = 200 μm. e Western blotting assay of cytoplasmic and nuclear expression of β-catenin in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3). f Measurement of luciferase activity in TOPflash-transfected and FOPflash-transfected hESCs treated with shMEN1 or shCtrl during decidualization (n = 3). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test.

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