Fig. 6: MEN1 knockdown aberrantly activated the WNT/β-catenin signaling pathway by downregulating SFRP2 and DKK1 expression. | Communications Biology

Fig. 6: MEN1 knockdown aberrantly activated the WNT/β-catenin signaling pathway by downregulating SFRP2 and DKK1 expression.

From: Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI

Fig. 6

a Heatmap of differentially expressed WNT signaling-related genes after MEN1 knockdown in decidualized hESCs (n = 3). b Western blotting assay of the H3K4me3 protein in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3); H3 served as a control. c qRT-PCR analysis of WNT signaling-related genes in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 0 and 4 days (n = 3). d Western blotting assay of SFRP2 and DKK1 expression in the shCtrl and shMEN1 groups (n = 3), GAPDH served as a control. e Western blotting assay of Menin, SFRP2, DKK1, IGFBP1, and H3K4me3 expression in the OE-Ctrl and OE-MEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3). GAPDH and H3 served as controls. f qRT-PCR analysis of SFRP2 and DKK1 expression in the OE-Ctrl and OE-MEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3). g Quantitative ChIP analysis of H3K4me3 at SFRP2 and DKK1 promoters in the shCtrl and shMEN1 groups treated with E2, MPA, and 8Br-cAMP for 4 days (n = 3). h qRT-PCR analysis of PRL and IGFBP1 expression in the shCtrl and shMEN1 groups treated with or without IWR-1. i Western blotting assay of Menin and IGFBP1 expression in the shCtrl and shMEN1 groups treated with or without IWR-1 on day 2 of decidualization. GAPDH served as a control. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test and one-way ANOVA.

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