Fig. 4: MAG phylogeny and relative abundance in the F-site cold seep water column. | Communications Biology

Fig. 4: MAG phylogeny and relative abundance in the F-site cold seep water column.

From: Methane filtration and metabolic cooperation of microbial communities in cold seep water columns from South China Sea

Fig. 4

A A phylogenomic tree derived from 377 MAGs based on 23,826 orthologous genes found by OrthoFinder. Their size, completeness, contamination, and relative abundance in each sample are indicated by different colors. Among the MAGs, the phyla Proteobacteria (n = 163, containing 84 Gammaproteobacteria and 27 Alphaproteobacteria), Actinobacteriota (n = 27), and Bacteroidota (n = 24) were most in bacteria, while Thermoplasmatota (n = 21) predominated in archaea. At a 95% ANI new species threshold, the MAGs show significant taxonomic novelty, with more than 81% of archaeal MAGs and 76% of bacterial MAGs representing new species, and 199/377 without a corresponding reference genome. The red branches represent Methylococcales, Thiotrichales, Nitrosococcales, Campylobacterales, and Bacteroidales; orders with higher abundance at the BWI. B Heat map illustrating the relative abundance of 18 Methylococcales MAGs across different sample sites, and the presence of the key genes involved in methylotrophic metabolism. The MAGs are labeled by their respective genera. Notably, the genus UBA1147 is classified under the family UBA2278, while all other genera belong to the family Methylomonadaceae. pmo particulate methane monooxygenase, mmo methane monooxygenase, mdh methanol dehydrogenase, xoxF lanthanide-dependent methanol dehydrogenase, hxl 3-hexulose-6-phosphate synthase, fae 5,6,7,8-tetrahydromethanopterin hydro-lyase, mtd methylenetetrahydromethanopterin dehydrogenase, mch methenyltetrahydromethanopterin cyclohydrolase.

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