Fig. 3: Identification of a cytosolic SpmX fragment to inhibit PodJ subcellular localization.
From: Construction and phenotypic classification of synthetic dual-pole Escherichia coli cells
a Domain organization of full-length SpmX and its transmembrane domain-truncated variant, SpmXΔTM. “IDR” denotes the intrinsically disordered region, and “TM” denotes the transmembrane domain. b Circuit diagrams illustrating the co-expression of sfGFP-PodJCC1−3, IDR with either mRFP (top) or mRFP-SpmXΔTM (bottom). sfGFP-PodJCC1−3, IDR was expressed under the control of the Ptac promoter, while mRFP and mRFP-SpmXΔTM were expressed from the AHL-inducible Plux promoter. c Images showing sfGFP-PodJCC1−3, IDR co-expression with either mRFP or mRFP-SpmXΔTM. mRFP or mRFP-SpmXΔTM were expressed under the control of the Plux promoter, and sfGFP-PodJCC1−3, IDR was expressed under the control of the Ptac promoter. Induction was performed at 28 °C with 10 μM AHL for 3 h, followed by 200 μM IPTG for 2 h. Scale bars: 1 µm. Fluorescence intensity profiles of sfGFP and mRFP along the long axis of the cell for the representative cell are shown on the right. d Fluorescence intensity (sfGFP) profiles along the long axis of the cell, analyzed from (c), with sfGFP-PodJCC1−3, IDR co-expressed with either mRFP (top) or mRFP-SpmXΔTM (bottom). Solid lines and colored belts represent the average intensity and standard deviation, respectively. n = 62 and 64 cells for mRFP and mRFP-SpmXΔTM co-expression, respectively.
