Fig. 1: CatB is expressed highly in the developing mouse cortex.
From: Cathepsin B deficiency disrupts cortical development via PEG3, leading to depression-like behavior
A CatB protein levels in mouse cortex at indicated embryonic (E), postnatal (P), or adult stages assessed by Western blot. Representative blots from three independent experiments (n = 3 mice per time point). B, C Quantification of pro-form (B) and mature-form (C) CatB expression normalized to Actin (n = 3 mice per group). D Schematic illustration of cell migration from ventricular zone (VZ) to preplate (PP) in E12.5 embryos. E Fluorescent images of CatB (magenta) and Hoechst (blue) in WT mice at E12.5, E14.5 and P0. Scale bar, 100 μm. F Quantification of CatB staining intensity in (E), normalized to VZ/SVZ at each time point. Images were taken from 3 mice per stage, 3 brain sections per mouse. G Immunofluorescent staining of CatB (magenta) with Ctip2 (green) or Trb1(green) in the cortex of WT mice at E12.5. Scale bar, 50 μm. *P < 0.05, **P < 0.01. Error bars represent mean ± SEM.
