Fig. 4: CatB knockout decreased the expression of development-related transcription factors.

A Similarity heatmap from 163 GO terms enriched in differentially expressed proteins by CatB KO that have been clustered and annotated with word clouds. B Representative developmental and differential related-GO terms enriched in differentially expressed proteins by CatB KO. C The volcano plot of the proteins identified from WT and CatB KO cortices by label-free quantitative LC-MS/MS. The proteins are plotted according to their log2 fold changes (x axis) and log10 P values (y axis). Proteins down-regulated and up-regulated in CatB KO are shown as blue and red dots, respectively. Among them, proteins with known functions in transcription and neuronal differentiation or nervous system development are marked. D Venn diagram of the number of differentially expressed proteins by CatB KO overlapping with transcriptional factors of mouse. The overlapping proteins related to cortical development and neuronal differentiation are labeled red. E Western blotting analysis of the expression of PEG3, Foxo1, and Foxc1 in cortex from E12.5 WT and CatB KO mice. F–H Quantification of the expression of PEG3 (F), Foxo1 (G), and Foxc1 (H) in cortex from E12.5 WT and CatB KO mice. PEG3 and Foxc1: n = 4 mice per group; Foxo1: n = 3 mice per group; all with 3 independent biological replicates. *P < 0.05, ***P < 0.001. Error bars represent mean ± SEM.