Fig. 1: OH2 can inhibit migration and invasion of NSCLC cell lines through MMP2, MMP7, and MMP9.

a Wound healing assay was performed on A549 and H1299 cells treated with or without OH2. Images shown are representative at time 0 h and 24 h post-treatment (left) and migration was quantified from images using ImageJ software, and graphs show means ± SEM from three independent experiments (right). b Transwell invasion assay was performed on A549 and H1299 cells treated with or without OH2 by the 24-transwell system. Representative photomicrographs of invading cells stained with crystal violet (left) and the number of invading cells and graphs show means ± SEM from three independent experiments (right). Scale bars, 100 μm. c A549 and H1299 cells were infected with or without OH2. Cells were lysed at 24 h post-infection and the samples were analyzed by western blotting. Western blot analysis (left) and quantification show means ± SEM from three independent experiments (right) of MMP2, MMP7, and MMP9 were performed. d Wound healing assay was performed on H1299 cells treated with or without GM6001. Data shown are means ± SEM from three independent experiments. e Transwell invasion assay was performed on H1299 cells treated with or without GM6001. Scale bars, 100 μm. Data shown are means ± SEM from three independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test in (a–e). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. f A549 and H1299 were incubated with or without OH2 and analyzed by CCK8 assay at various time points. Data are presented as means ± SD.