Fig. 10: PA modulates the TGF-β/SMAD signaling pathway via IGFBP6. | Communications Biology

Fig. 10: PA modulates the TGF-β/SMAD signaling pathway via IGFBP6.

From: Pantothenic acid ameliorates hepatic fibrosis by targeting IGFBP6 to regulate the TGF-β/SMADs pathway

Fig. 10

Phospho-SMAD2/3 levels detected by Western blotting in (a) control group and (b) IGFBP6-knockout group treated with TGF-β (10 ng/mL) for 15 min, 30 min, or 60 min with or without PA (10 µM) (n = 3). Data are expressed as means ± SD, One-way ANOVA. c Immunofluorescence analysis of TGF-β (10 ng/mL) -induced SMAD2/3 nuclear translocation in LX2 cells with or without IGFBP6 knockout, treated with or without PA (10 µM) (scale bar: 25 μm). d The mRNA expression levels of Acta2, Fn1, and Col1a1 in primary mouse HSCs in the presence or absence of PA or the SMAD3 inhibitor SIS3 were detected by qRT-PCR (n = 3). Data are expressed as means ± SD, Two-way ANOVA.

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