Fig. 5: Father companionship changed the gut microbiota of male offspring.

a The ACE index, Chao1 index, Shannon index, and PD-whole-tree index indicate that father’s companionship and control groups did not show differences in α-diversity. b PCoA plot indicated the difference in β-diversity among male mice. c Relative abundance of gut microbial community at the genus level. d Linear discriminant analysis (LDA) effect size (LEfSe) analysis for taxonomic composition gut microbiota. e–g Correlation between the relative abundance of Bilophila and defensive attack behavior. The relative abundance of Bilophila was positively associated with the frequency of biting (r = 0.757, P = 0.006). h–j Correlation between the relative abundance of Bifidobacterium and defensive attack behavior. The relative abundance of Bifidobacterium was negatively associated with the frequency of biting (r = -0.690, P = 0.016). FC Father companionship.