Fig. 1: OPTN loss-of-function leads to RGC loss and astrogliosis.
From: OPTN protects retinal ganglion cells and ameliorates neuroinflammation in optic neuropathies
a Schematic illustration of the OPTN ablation in retina. Created with BioRender.com and used with permission. b Representative IF images showing OPTN knockout induced RGC loss and astrogliosis 1 month post intravitreal injection. Scale bar, 50 μm. c Quantification of RGC survival with OPTN knockout 1, 3, and 6 months post intravitreal injection. Data are shown as mean ± s.e.m. with n = 5 biologically independent samples. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. d IOP of mice post intravitreal injection of AAV2-Cre or PLAP virus shows no significant difference between OPTN knockout and control mice. Data are shown as mean ± s.e.m. with n = 10 biologically independent samples. e Quantification of astrogliosis with OPTN knockout 1, 3, and 6 months post intravitreal injection. Data are shown as mean ± s.e.m. with n = 5 biologically independent samples. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test for multiple comparisons.
