Fig. 2: OPTN loss-of-function leads to microglia activation.
From: OPTN protects retinal ganglion cells and ameliorates neuroinflammation in optic neuropathies
a Representative IF images showing OPTN knockout induced microglia activation 6 months post intravitreal injection. Scale bar, 50 μm. b A lateral view of 3D-reconstituted section retina with OPTN knockout 3 months post intravitreal injection. Scale bar, 5 µm. c Quantification of percentage of Iba1-positive microglia that are also CD68-positive with OPTN knockout 1, 3, and 6 months post intravitreal injection. Data are shown as mean ± s.e.m. with n = 5 biologically independent samples. ***p < 0.001, ns, not significant, calculated by unpaired t-test. d–f Quantification of the number of Iba1-positive microglia (d), Iba1-positive microglia cell body diameter (e), and the average primary process length of Iba1-positive microglia (f) at 1, 3, and 6 months post intravitreal injection. Data are shown as mean ± s.e.m. with n = 5 biologically independent samples. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test for multiple comparisons.
