Fig. 2: Growth patterns and initial telomere length variations in postnatal offspring born from mouse blastocysts in vivo and in vitro. | Communications Biology

Fig. 2: Growth patterns and initial telomere length variations in postnatal offspring born from mouse blastocysts in vivo and in vitro.

From: NAD+-dependent Sirt6 is a key regulator involved in telomere shortening of in vitro-cultured preimplantation embryos

Fig. 2

a Representative image of F1 live births by uterus transplantation of the blastocysts in vivo or in vitro. b Sex ratio pie chart of mouse F1 pups in the in vivo (n = 13) and in vitro (n = 6) groups. c Body weights of mouse F1 offspring monitored at 1 week, 3weeks, 5weeks, 7 weeks, 9 weeks, 11 weeks of age in the in vivo (n = 13) and in vitro (n = 6) groups. d Relative telomere length of mouse F1 pups in the in vitro (n = 13) and in vitro (n = 6) groups at 1 week, 3 weeks, 5 weeks, 7 weeks, 9 weeks, 11 weeks of age, respectively. Relative telomere length is expressed as a T/R ratio determined by qPCR analysis. e Body weights of mouse F2 offspring (self-mating of F1 females with F1 males within each group) monitored at 1 week, 3weeks, 5weeks, 7 weeks, 9 weeks, 11 weeks of age in the in vivo (n = 24) and in vitro (n = 20) groups. f Relative telomere length of mouse F2 pups in the in vivo (n = 24) and in vitro (n = 20) groups at 1 week, 3 weeks, 5 weeks, 7 weeks, 9 weeks, 11 weeks of age, respectively. Relative telomere length is expressed as a T/R ratio determined by qPCR analysis. All the data are expressed as the median (interquartile range, IQR) and means ± standard deviations (SDs). * P < 0.05; ****P < 0.0001; ns: not significant, P > 0.05 (Two-way ANOVA).

Back to article page