Fig. 1: Overview of the co-culture system and pipelines.
From: Deciphering antigen-specific T cell navigation tactics and cancer immune evasion in co-cultures
A Schematic representation illustrating the integrated workflow for experimental set-up, motility analysis, and gene expression profiling. B Diagrams of the co-culture experiments and representative 2D microscopic images for the WT and OVA groups. Scale bars, 100 µm. C Quantification of the number of recruited CTLs on cancer cells over time for WT or OVA groups, manually counted within a 15 µm surrounding region every 30 min (see Methods). For each time point, the total number of CTLs residing on cancer cells within the imaging field was counted (n = 28 for the OVA groups and n = 9 for the WT groups from one independent experiment, mean ± s.e.m.). D Counts of live cancer cells over time in different regions of the imaging field for the WT and OVA groups (n = 9, mean ± s.e.m.). E Interaction time of CTLs on cancer clusters for B16-OVA and B16-WT groups (n = 217 vs. 225). C–E Statistical significance was determined using the Two-sided Wilcoxon rank-sum test: p < 0.001 (***), p < 0.01 (**), p < 0.05 (*), p > 0.05 (n.s.). For (C), the p values at t = 0 h, 6 h, 12 h, and 18 h were 2.13E-01, 8.26E-02, 4.96E-05 and 3.72E-04, respectively. For (D), the p values at t = 0 h, 6 h, 12 h, and 18 h were 3.32E-02, 5.22E-03, 4.11E-05 and 4.11E-05, respectively. For (E), the p-value is 4.46E-41.
