Fig. 2: Quantitative motility patterns of CTLs in the co-culture with OVA and WT cancer cells.
From: Deciphering antigen-specific T cell navigation tactics and cancer immune evasion in co-cultures
A Pipeline for processing time-lapse images and tracking T cells. B Schematic representation of data analysis, including dwell time, mean square displacement, directional bias, directional persistence of CTLs towards cancer cell clusters. C Density of CTLs on cancer clusters (cells/mm2) over time, demonstrating a significant T cell accumulation in OVA groups compared to WT groups (n = 5). Statistical significance was determined using the Two-sided Wilcoxon rank-sum test: p < 0.001 (***), p < 0.01 (**), p < 0.05 (*), p > 0.05 (n.s.). The p-values at 0 h, 6 h, 12 h, and 18 h were 1, 1, 7.94E-03 and 7.94E-03, respectively. D Dwell time distribution of T cells on cancer-cell clusters in Series 1, divided into 6-h intervals. The left panel (0–6 h) shows n = 149 adhesion events for the WT groups and n = 110 events for the OVA groups; the right panel (6–12 h) shows n = 131 events for the WT groups and n = 223 events for the OVA groups. The distribution for 12–18 h is shown in Supplementary Fig. 3D. E Step size distributions of CTLs over 6-hour intervals: Left panel (0–6 h), middle panel (6–12 h), and right panel (12–18 h). The p-values for (E) (left, middle, and right) are 9.9E-23, 3.3E-26 and 1.8E-70, respectively. Data represent results from n = 5 independent experiments. D, E Statistical significance was determined using the Two-sided two-sample Kolmogorov–Smirnov test. KS statistic D and p-value are shown inside the plotting boxes. F Directional bias and G persistence distributions of CTLs movement towards the nearest cancer cell boundary within three time-intervals. Statistical significance was determined using the two-sided unpaired Student’s t test with p < 0.001 (***), p < 0.01 (**), p < 0.05 (*). [a, b) defines the value range where x ≥ a and x < b. C–G Red lines (or bars) represent OVA groups, and cyan lines (or bars) represent WT groups. C, F, G, n = 5 independent experiments, mean±s.e.m. The p-values for (F, G) are provided in Supplementary Data 8.
