Table 1 Langmuir derived Equilibrium and kinetic binding terms for AP2 to different biomimetic PM

From: Combined thermodynamic and time-resolved structural analysis of interactions between AP2 and biomimetic plasma membranes provides insights into clathrin-mediated endocytosis

 

Kinetic binding analysis

Equilibrium data Π = 25mN/m

 

Kon /104 s−1 M−1

Koff

/10-4 s−1

Residence time / s

Kd/nM

Kd/nM

β

Kd’ (*) /nM

β‘(*)

PtdIns(4,5)P2

7.9 ± 0.7

5.6 ± 0.5

1785

7 ± 1

5 ± 1

0.9 ± 0.1

  

TGN+ PtdIns(4,5)P2

1.5 ± 0.1

1.8 ± 0.1

5555

12 ± 1

15 ± 3

1.0 ± 0.1

25 ± 6

1.1 ± 0.1

CD4+ PtdIns(4,5)P2

65 ± 2

1.2

8333

18 ± 3

29 ± 5

1.1 ± 0.1

75 ± 9

1.9 ± 0.4

  1. (*) After contribution of PtdIns(4,5)P2 subtracted.
  2. β > 1 indicates a cooperative scenario according to the Hill equation.
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