Fig. 2: kmt2d knockout zebrafish showed defects in social behaviors.
From: Deficiency of KMT2D causes autistic-like behavior in mice and zebrafish
A–C Diagram of zebrafish kmt2d gene and mutation induced by CRISPR/Cas9, gray boxes indicate exons. A target site was on Exon 28 resulting in a 92-base deletion and 6-base insertion. B Sanger sequence. C results of agarose gel electrophoresis. D kmt2d−/− embryos exhibiting morphological abnormalities at the age of 24 h, 36 h and 72 h. E The expression levels of the Kmt2d gene decreased at three developmental point for embryos exhibiting KS phenotypes (Fifteen embryos per biological replicate were collected for zebrafish RT-qPCR, n = 3). F Body length was reduced in the kmt2d knockout zebrafish (n = 9). G Schematic diagram of the social preference behaviours experiment and motion trajectory chart for zebrafish. H Sniffing time exploring social region (n = 6 for wt, n = 7 for kmt2d-/+). I The distance traveled in the mating tank, which suggested that the kmt2d-/+ zebrafish had normal motor skill (n = 6 for wt, n = 7 for kmt2d−/+). Error bars represent as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ns: P > 0.05, Mann-Whitney U test.
