Fig. 3: Kmt2d deficiency mice showed defects in social behaviors and had repetitive behavior.
From: Deficiency of KMT2D causes autistic-like behavior in mice and zebrafish
A The adeno-associated virus (AAV) vectors carrying Kmt2d-shRNA (AAV-RNAi) or control-shRNA was delivered to the hippocampal. B The results of RT-qPCR showed a significantly reduced of kmt2d in the AAV-RNAi group compared to that of AAV-nc group (n = 3). C, D The level of the H3K4me3 protein. The band density was quantified and expressed as the relative gray value (compared with the control) by arbitrarily setting the control value as 1 (n = 3). E Schematic diagram of the three-box social experiment. F The distance traveled in different areas within the open field for the mice (n = 6). G Motion trajectory chart for the control (AAV-nc) and knockdown (AAV-RNAi) mice in an Open Field Assay. H Number of marbles buried (n = 6). I, J During a social interaction session, Kmt2d deficiency mice didn’t have social preferences. I sniffing time exploring S1 and E (n = 6). J motion trajectory chart for the control (AAV-nc) and knockdown (AAV-RNAi) mice. K, L The same as (I, J) but for social novelty test. K sniffing time exploring S1 and S2. (n = 6). L motion trajectory chart. Error bars represent as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ns: P > 0.05, Mann-Whitney U test. A Created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
