Fig. 4: Knockdown of Kmt2d decreased excitatory and increased inhibitory synaptic transmission.
From: Deficiency of KMT2D causes autistic-like behavior in mice and zebrafish
A Schematic diagram of hippocamcal cultured slice and dual whole-cell patch clamp. B Paired recordings from Kmt2d-shRNA-eGFP infection (bright cell) and control cell. C, D The amplitude of IPSCs in infected cells is increased compared to control cells in CA1 and DG regions, respectively (n = 10 for CA1 region; n = 9 for DG region). E, G The amplitude of AMPA receptor-mediated EPSCs in infected cells is decreased compared to control cells in CA1 and DG regions, respectively (n = 10 for CA1 region; n = 11 for DG region). F, H The amplitude of NMDA receptor-mediated EPSCs in infected cells remained unchanged compared to control cells in CA1 and DG regions, respectively (n = 9 for CA1 region; n = 11 for DG region). Error bars represent as mean ± SD. *P < 0.05, **P < 0.01, Mann-Whitney U test. A Created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
