Fig. 3: Neutrophils are redundant in the development of neuroinflammation.
a–e Peripheral myeloid cells were depleted by the intraperitoneal injection of anti-Ly6C/Ly6G antibody (10 µg/g) on day -1 and day 0 of photothrombotic (PT) stroke induction. Myeloid-competent mice received an equivalent dose of IgG2b (IgG) control. a Gating strategy used for flow cytometry analyses of red blood cell-lysed blood to confirm myeloid cell depletion. Blood immune populations were further analyzed for b neutrophils (CD45hiCD11b+Ly6G+) and c monocytes (CD45hiCD11b+Ly6G−Ly6C+). d Perfused brains were homogenized, and tissue supernatant was analyzed using a LEGENDplex inflammatory cytokine panel. Analytes taken from infarct core homogenates were measured for cytokines and presented as log2 fold-change over contralateral (CL) control (IgG n = 6, Ly6C/G n = 8). e Time taken for mice to fall from a suspended wire. Graphs are presented as mean ± SD. An unpaired t test was used when comparing 2 groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns no significance.
