Fig. 5: Rapid morphological response of microglia to photothrombotic stroke.
Cx3cr1gfp/+ mice were subjected to a photothrombotic (PT) stroke for a–f 3 or g–l 24 h. Brain sections (30 µm) were immunofluorescently stained with anti-GPIbβ (platelets; cyan) and anti-laminin (vasculature; red) to complement the microglia green fluorescent protein (GFP)-reporter (green) and Z-stack images (0.5 µm intervals) were acquired using an Olympus FVMPE-RS two-photon microscope. a, g Representative images were taken at regions across the brain, relative to stroke location. Raw Z-compressed images were 3-D rendered (using IMARIS v10) for morphological analysis. CX3CR1+ microglia were assessed for b, h count per field of view (FOV), c, i cell body volume, d, j cell body sphericity, e, k dendrites per cell and f, l dendrite complexity (n = 5 mice; individual cells are plotted for volume and sphericity [≈n = 500/region]). A repeated measures one-way ANOVA was used for analysis of multiple groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns no significance.
