Fig. 2: Deficiency of Tex38 caused oligoasthenozoospermia in male mice.
From: Disruption of TEX38 impairs sperm morphogenesis and the migration of sperm into the oviduct
A H&E staining of the caput epididymis, corpus epididymis, and cauda epididymis from Tex38+/+ mice and Tex38−/− mice. B Count of the sperm from cauda epididymis (n = 3). Student’s t-test. C The percentage of motile spermatozoa in the cauda epididymis (n = 3). Student’s t-test. D The percentage of the sperm with progressive motility in the cauda epididymis (n = 3). Student’s t-test. E H&E staining of spermatozoa from the cauda epididymis. F The percentage of normal sperm, bent neck sperm, malformed head sperm, and multi-headed/flagellated sperm in Tex38+/+ and Tex38−/− cauda epididymis (n = 3). Student’s t-test. G Scanning electron microscopy (SEM) images of sperm from Tex38+/+ and Tex38−/− mice. H TEM analysis of mature sperm in the Tex38+/+ and Tex38−/− cauda epididymis. The red arrow indicates the absence and disorder of microtubules in the sperm flagella of Tex38−/− mice. I Peanut agglutinin (PNA) staining of epididymal sperm from Tex38+/+ and Tex38−/− mice. Scale bar is 25 μm. J Quantification of the percentage of PNA positive sperm in Tex38+/+ and Tex38−/− mice (n = 3, more than 200 sperm per replicate). Student’s t-test. K, L Comet assay for DNA fragmentation of epididymal sperm from Tex38+/+ and Tex38−/− adult mice (n = 9, more 500 sperm per replicate). Scale bar is 50 μm. Student’s t-test.
