Fig. 5: In vitro fertilization assessment of sperm from Tex38−/− mice.
From: Disruption of TEX38 impairs sperm morphogenesis and the migration of sperm into the oviduct
A Sperm from Tex38+/+ and Tex38−/− mice was induced by calcium ionophore A23187 supplementation and PNA staining was performed. Nuclei were counterstained with DAPI. Representative images were shown. Scale bar is 25 μm. B Quantification of the percentages of acrosome reaction (AR) in (A) (n = 3, more than 200 sperm per replicate). Student’s t-test. C In vitro analysis of sperm–egg binding. Sperm heads were stained with Hoechst 33342. Scale bar is 50 μm. D ATP contents in Tex38+/+ and Tex38−/− sperm (n = 3). Student’s t-test. E Zona pellucida (ZP) intact oocytes after 24 h of in vitro fertilization (IVF) with epididymal sperm from adult Tex38+/+ or Tex38−/− mice. Scale bar is 100 μm. F Quantification comparison of 2-cell rate following IVF of Tex38+/+ ZP intact oocytes with epididymal sperm from adult Tex38+/+ or Tex38−/− mice (n = 3). Student’s t-test. G ZP free oocytes after 24 h of IVF with epididymal sperm from adult Tex38+/+ or Tex38−/− mice. Scale bar is 100 μm. H Quantification comparison of 2-cell rate following IVF of Tex38+/+ ZP free oocytes with epididymal sperm from adult Tex38+/+ or Tex38−/− mice (n = 4). Student’s t-test. Data are mean ± SEM and dots represent values for individual mice.
