Fig. 7: ARRDC5 exhibits similar characteristic to its interacting protein TEX38 during spermatogenesis.
From: Disruption of TEX38 impairs sperm morphogenesis and the migration of sperm into the oviduct
A Screen the interacting proteins of TEX38 in STRING database (https://cn.string-db.org). B ARRDC5 interacted with TEX38 in cultured HEK293T cells. C Protein expression of ARRDC5 in the Tex38+/+ and Tex38−/− testis, epididymis, and sperm. D RT-PCR analysis of Arrdc5 mRNA in mice tissue. Rps2 was used as loading control. E Western blot of ARRDC5 protein in mice tissue. β-actin was used as a control. F RT-PCR analysis of Arrdc5 mRNA in mice developing testis. G Western blot of ARRDC5 protein in mice developing testis. H Protein expressions of ADAM3, CALR3, CLGN, PDILT, TEX38 in Arrdc5+/+ and Arrdc5−/− testis and sperm. I Quantitation of protein expressions in (H) (n = 3). Mann-Whitney U test.
