Fig. 3: pa-BAK knockdown is correlated with decreased DNA damage and ROS in coral host cells.
From: BAK knockdown delays bleaching and alleviates oxidative DNA damage in a reef-building coral
A The level of oxidative DNA damage marker (8-OHdG) in DNA of heat-stressed corals with successful pa-BAK knockdown (siBAK) and controls of the same colony (siNTC; n = 7). B Correlation between the tolerance gain (calculated as the percent difference between bleaching rates of treated (siBAK) and control corals (siNTC)) and the relative decrease of oxidative DNA damage marker in treated corals (n = 7). C The level of ROS in coral host cells, calculated as the mean fluorescent signal normalized by background signal. AT stands for ambient temperature; siBAK and siNTC underwent heat stress. D Example of microscopy images of each cell type captured in each channel. Hoechst 33342 stains DNA. CellRox becomes fluorescent after oxidation and is used as a proxy for reactive oxygen species (ROS) accumulation over time. Host cells (right side of the panel) are in three different categories based on the GFP signal.
