Fig. 5: Mutation to the basic residues in IL3 in the TMD abolishes the inhibition of NBCe1-A by P-loop.

a Diagram showing the spatial arrangement of TMs in NBCe1 TMD (bottom view). Yellow: TMs in the carrier domain. Green: TMs in the scaffold domain. b Localization of basic residues in IL4 in the carrier (yellow), and IL3 and IL6 in the scaffold (green). c Effect of mutations to the basic residues in IL3 on the activity of NBCe1-A with either P-loop^WT or P-loop^12D. d Summary of percentile inhibition of NBCe1-A by P-loop^12D. The figure was created based on the data contributed to c. e Proposed model for the mechanism of NBCe1-A inhibition by phosphorylation of P-loop. Data are presented as mean ± SD. The numerals in the parentheses on the bars indicate the number of individual oocytes. ns, not significant by two-tailed student’s t-test. In d, one-way ANOVA followed by post-hoc Dunnett’s multiple comparisons was used for statistical analyses. ****p < 0.0001.