Fig. 3: ROS accumulation stimulates FOXO activity. | Communications Biology

Fig. 3: ROS accumulation stimulates FOXO activity.

From: The ROS–FOXO pathway mediates broad-spectrum detoxification of acaricides in Tetranychus cinnabarinus

Fig. 3

a Structural analysis of the T. cinnabarinus FOXO protein. Forkhead denotes the DNA-binding domain, NLS represents a putative nuclear localization signal, and TAD refers to the transactivation domain. b qPCR analysis of FOXO mRNA expression in T. cinnabarinus pretreated with NAC after fenpropathrin treatment. c qPCR analysis of FOXO mRNA expression in T. cinnabarinus pretreated with NAC after cyflumetofen treatment. d qPCR analysis of FOXO mRNA expression in T. cinnabarinus pretreated with NAC after chlorpyrifos treatment. In b–d tween 80 in water as a control and NAC as a treatment were used to pretreat adult female mites for 24 h before dividing the mites into two groups: one treated with acetone for 24 h and the other treated with acaricide for 24 h. Error bars represent the mean ± SE, n = 3. Asterisks indicate a statistically significant difference analyzed by Student’s t-test. *p < 0.05. e qPCR analysis of FOXO mRNA expression in T. cinnabarinus after paraquat treatment. The control was treated with acetone and used for normalization. Error bars represent the mean ± SE, n = 3. Lowercase letters represent statistically significant differences, analyzed by one-way ANOVA (Tukey’s HSD), p < 0.05. f qPCR analysis of FOXO mRNA expression in T. cinnabarinus pretreated with NAC after paraquat treatment. Error bars represent the mean ± SE, n = 3. Asterisks indicate a statistically significant difference, as analyzed by Student’s t-test. *p < 0.05. g Western blot analysis of FOXO protein expression after paraquat treatment. α-tubulin was used as the loading control. h Western blot analysis of FOXO protein distribution after paraquat treatment. CK1 indicates the untreated group, CK2 indicates the 0.1% Tween 80 water treatment for 24 h, and paraquat indicates the paraquat treatment for 24 h. α-tubulin and histone H3 were used as cytoplasmic and nuclear loading controls, respectively. The values in g, h represent the ratio of the TcFOXO protein band density relative to tubulin or histone H3.

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