Fig. 5: Expression of detoxification genes regulated by CncC, AhR, and TcHR96f elucidated by RNAi and DAP-seq. | Communications Biology

Fig. 5: Expression of detoxification genes regulated by CncC, AhR, and TcHR96f elucidated by RNAi and DAP-seq.

From: The ROS–FOXO pathway mediates broad-spectrum detoxification of acaricides in Tetranychus cinnabarinus

Fig. 5

a qPCR analysis of CncC mRNA expression after dsCncC feeding. b qPCR analysis of AhR mRNA expression after dsAhR feeding. c qPCR analysis of TcHR96f mRNA expression after dsTcHR96f feeding. A down arrow indicates expression silencing after interference, with silencing efficiency shown as a percentage. d Measurement of T. cinnabarinus sensitivity toward fenpropathrin, cyflumetofen, and chlorpyrifos after CncC, AhR, and TcHR96f silencing. The Y-axis represents mortality rate. In a–d error bars represent the mean mortality rate ± SE, n = 3. Asterisks indicate a statistically significant difference (dsCncC, dsAhR, or dsTcHR96f vs. dsGFP), as analyzed by Student’s t-test. *p < 0.05, **p < 0.01. e Distribution of CncC peaks in functional genomic elements. f Promoter binding sequence motifs of the T. cinnabarinus CncC predicted by DAP-seq. g Distribution of AhR peaks in functional genomic elements. h Promoter binding sequence motifs of T. cinnabarinus AhR predicted by DAP-seq. i Distribution of TcHR96f peaks in functional genomic elements. j Promoter binding sequence motifs of TcHR96f predicted by DAP-seq.

Back to article page