Fig. 6: Functional analysis of CYP392E3, CYP388A1, TcGSTm8, and TcCCE34. | Communications Biology

Fig. 6: Functional analysis of CYP392E3, CYP388A1, TcGSTm8, and TcCCE34.

From: The ROS–FOXO pathway mediates broad-spectrum detoxification of acaricides in Tetranychus cinnabarinus

Fig. 6

a qPCR analysis of the expression of eight detoxification genes after fenpropathrin treatment. b qPCR analysis of the expression of eight detoxification genes after cyflumetofen treatment. c qPCR analysis of the expression of the eight genes after chlorpyrifos treatment. In a–c, controls were treated with acetone and used for normalization. Data was expressed as the mean, n = 3. Asterisks indicate a statistically significant difference, analyzed by Student’s t-test, *p < 0.05. d qPCR analysis of CYP392E3 mRNA expression after dsCYP392E3 feeding. e qPCR analysis of CYP388A1 mRNA expression after dsCYP388A1 feeding. f qPCR analysis of TcGSTm8 mRNA expression after dsTcGSTm8 feeding. g qPCR analysis of TcCCE34 mRNA expression after dsTcCCE34 feeding. A down arrow indicates expression silencing after interference, with silencing efficiency shown as a percentage. h The sensitivity of T. cinnabarinus to fenpropathrin, cyflumetofen, and chlorpyrifos after CYP392E3, CYP388A1, TcGSTm8, and TcCCE34 silencing. The Y-axis represents mortality rate. In d–h, error bars represent the mean ± SE, n = 3. Asterisks indicate a statistically significant difference, analyzed by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. i Recombinant TcGSTm8 metabolizes cyflumetofen. CK1: recombinant protein + 10 mg L−1 cyflumetofen, reaction for 0 h; CK2: inactivated recombinant protein + 10 mg L−1 cyflumetofen, reaction for 1 h; CK3: PBS without recombinant protein + 10 mg L−1 cyflumetofen, reaction for 1 h. j Recombinant CYP388A1 metabolizes fenpropathrin. CK is fenpropathrin + recombinant CYP388A1 – NADPH. k Recombinant CYP388A1 metabolizes cyflumetofen. CK1: recombinant CYP388A1 + NADPH + 10 mg L−1 cyflumetofen, reaction for 0 h; CK2: inactivated recombinant CYP388A1 + NADPH + 10 mg L−1 cyflumetofen, reaction for 3 h; CK3: PBS without recombinant CYP388A1 + 10 mg L−1 cyflumetofen, reaction for 3 h. l Recombinant CYP388A1 degrades chlorpyrifos. CK is chlorpyrifos + recombinant CYP388A1 – NADPH. m Recombinant TcCCE34 metabolizes fenpropathrin. CK is fenpropathrin + recombinant inactivated TcCCE34, reaction for 3 h. In i–m The Y-axis represents the acaricide concentration, and error bars represent the mean ± SE, n = 3. Asterisks indicate a statistically significant difference, analyzed by Student’s t-test. **p < 0.01, ***p < 0.001. A down arrow indicates that chlorpyrifos is metabolized, with catabolic efficiency expressed as a percentage.

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