Fig. 1: Detection of fibrosis and identification of core genes. | Communications Biology

Fig. 1: Detection of fibrosis and identification of core genes.

From: Therapeutic potential of Desmodium styracifolium polysaccharide in attenuating nano-calcium oxalate induced renal injury and fibrosis

Fig. 1

A Immunofluorescence double staining and quantification of relative fluorescence intensity of α-SMA in kidney tissues from clinical kidney stone patients. (Green: CK-18, epithelial cell marker; Red: α-SMA, Blue: DAPI). Magnification is×400, Scale bar = 20 μm. Data are mean ± SD, n = 3, one-way ANOVA. B Masson staining of rat kidneys and calculation of the percentage of collagen volume fraction in the kidney stone model (collagen blue area/total tissue area × 100%); Magnification is ×200, Scale bar = 100 μm. Data are mean ± SD, n = 3, one-way ANOVA. C Volcano plots displaying differential gene expression between the normal control and kidney stone model groups in rats. n = 3. D Venn plots illustrating the overlap between differential genes in the rat model and fibrosis-related genes from databases. E Diagram depicting the protein interactions among differential genes, with connecting lines indicating interactions between the proteins. F Cytoscape calculation of core genes, with darker colors indicating higher gene scores. Compared with the Normal or Control group, ** indicates P < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001.

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