Fig. 6: Ds inhibits nano-COM-induced cellular oxidative damage. | Communications Biology

Fig. 6: Ds inhibits nano-COM-induced cellular oxidative damage.

From: Therapeutic potential of Desmodium styracifolium polysaccharide in attenuating nano-calcium oxalate induced renal injury and fibrosis

Fig. 6

A ROS, JC-1, and PI staining of HK-2 cells. In JC-1 staining, red represents JC-1 aggregates and green represents JC-1 monomers. Magnification is ×200, Scale bar = 100 μm. n = 3. B LDH kit quantification. Data are mean ± SD, n = 4, one-way ANOVA. C MDA kit quantification. Data are mean ± SD, n = 3, one-way ANOVA. D Relative quantification of ROS fluorescence. Data are mean ± SD, n = 3, one-way ANOVA. E Relative Fluorescence intensity ratio of mitochondrial membrane potential. Data are mean ± SD, n = 3, one-way ANOVA. F Counting of PI-stained cells. Data are mean ± SD, n = 3, one-way ANOVA. G Immunoblotting analysis of NRF-2 and KEAP-1. n = 3. H Semi-quantitative chart of KEAP-1 and NRF-2 expression. Data are mean ± SD, n = 3, one-way ANOVA. Compared with the COM Group, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001.

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