Fig. 7: Ds protects the epithelial phenotype of HK-2 cells. | Communications Biology

Fig. 7: Ds protects the epithelial phenotype of HK-2 cells.

From: Therapeutic potential of Desmodium styracifolium polysaccharide in attenuating nano-calcium oxalate induced renal injury and fibrosis

Fig. 7

A Immunofluorescence double staining of HK-2 cells (Green: CK-18; Red: Vimentin; Blue: DAPI). Magnification is ×400, Scale bar = 20 μm. n = 3. B Relative quantification of fluorescence expression of Vimentin. Data are mean ± SD, n = 3, one-way ANOVA. C Detection of TGF-β expression in culture media by Elisa Kit. Data are mean ± SD, n = 4, one-way ANOVA. D TGF-β protein expression in HK-2 cells stimulated by gradient concentrations of nano-COM. n = 3. E Nano-COM induces changes in TGF-β and N-cadherin protein expression in HK-2 cells at gradient times. n = 3. F Changes in the expression of fibrosis-related proteins and TGF-β/Smad pathway-related proteins. n = 3. G–J Semi-quantitative statistical graphs of TGF-β, E-cadherin, N-cadherin, Vimentin, Smad2/3, P-Smad2, and P-Smad3. Data are mean ± SD, n = 3, one-way ANOVA. K–M RT-qPCR analysis of TGF-β, E-cadherin, and Vimentin. Data are mean ± SD, n = 3, one-way ANOVA. Compared with the COM Group, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001.

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