Fig. 9: Human recombinant protein TGF-β reverses the effects of Ds. | Communications Biology

Fig. 9: Human recombinant protein TGF-β reverses the effects of Ds.

From: Therapeutic potential of Desmodium styracifolium polysaccharide in attenuating nano-calcium oxalate induced renal injury and fibrosis

Fig. 9

A Cell morphology of HK-2 cells observation by phase-contrast microscope. Magnification is ×100, Scale bar = 200 μm. n = 3. B ROS, JC-1, and PI staining of HK-2 cells. Magnification is ×100, Scale bar = 200 μm; ×200, Scale bar = 100 μm. n = 3. C Relative quantification of ROS fluorescence. Data are mean ± SD, n = 3, one-way ANOVA. D Relative Fluorescence intensity ratio of mitochondrial membrane potential. Data are mean ± SD, n = 3, one-way ANOVA. E Counting of PI-stained cell. Data are mean ± SD, n = 3, one-way ANOVA. F Immunofluorescence double staining of HK-2 cells (Green: CK-18; Red: Vimentin; Blue: DAPI) and relative quantification of Vimentin fluorescence intensity; Magnification is ×400, Scale bar = 20 μm. Data are mean ± SD, n = 3, one-way ANOVA. G Immunoblotting analysis of NRF-2 and Vimentin. n = 3. H, I Semi-quantitative statistical graphs of proteins. Data are mean ± SD, n = 3, one-way ANOVA. J, K RT-qPCR analysis of E-cadherin and Vimentin. Data are mean ± SD, n = 3, one-way ANOVA. Compared with the COM group, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. Compared with the COM + Ds Group, ## indicates p < 0.01, ### indicates p < 0.001, #### indicates p < 0.0001, and ns indicates p > 0.05, with no statistical difference.

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