Fig. 4: SNHG3 c.1746 A > I editing enhances PA utilization to promote FAO. | Communications Biology

Fig. 4: SNHG3 c.1746 A > I editing enhances PA utilization to promote FAO.

From: A-to-I edited SNHG3 promotes non-small cell lung cancer metastasis by promoting fatty acid oxidation and resisting ferroptosis

Fig. 4

a Increased palmitoylcarnitine levels in SNHG3ED versus SNHG3WT cells after PA treatment (n = 6, unpaired t-test). b Enhanced FAO activity (FAOBlue fluorescence) following PA stimulation (scale bar=20μm). Seahorse analysis showing differential OCR (c) and respiratory capacity (d) responses to PA and etomoxir (Eto) treatment (n = 4, one-way ANOVA). e Metabolic tracing schematic of 13C-PA through β-oxidation and TCA cycle (red indicates 13C-labeled carbons). Metabolic profiling of Palmitoylcarnitine (f), Acetyl-CoA (g), Citrate (h), Succinate (i), and Acetoacetate (j) in response to 13C-PA treatment (n = 4, unpaired t-test). Data are mean ± SD. Significance: *P < 0.05, **P < 0.01, ***P < 0.001; ns not significant. N indicates biological replicates.

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