Fig. 5: c.1746 A > I editing strengthens SNHG3-SSRP1 interaction. | Communications Biology

Fig. 5: c.1746 A > I editing strengthens SNHG3-SSRP1 interaction.

From: A-to-I edited SNHG3 promotes non-small cell lung cancer metastasis by promoting fatty acid oxidation and resisting ferroptosis

Fig. 5

a Subcellular localization of SNHG3 by qPCR using NEAT (nuclear) and GAPDH (cytoplasmic) markers. Solid black circles denote cytoplasmic expression levels, while large open circles represent nuclear expression values. b RNA pull-down mass spectrometry showing differential protein binding to SNHG3ED versus SNHG3WT. c Western blot validation of seven candidate nuclear proteins from pull-down assays. d RIP assays confirming enhanced SNHG3ED-SSRP1 binding (n = 3-4, unpaired t-test). e Immunofluorescence showing SNHG3 (red) and SSRP1 (green) nuclear co-localization (scale bar = 10μm), and colocalisation threshold analysis. f SSRP1 binding to full-length versus Δ1500-1900 truncated SNHG3 by RNA pull-down. g qPCR analysis of target genes after SSRP1 knockdown (n = 3, unpaired t-test). Transwell migration (h; Scale bar = 50 μm) and invasion (i; Scale bar = 50μm) assays post-SSRP1 inhibition (n = 3, unpaired t-test). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001; ns = not significant. N indicates biological replicates.

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