Fig. 4: Neonatal injection of AAV-dNmCas9/Nmg15 improves hind limb clasp phenotype in AS mice.
From: AAV-dCas9 vector unsilences paternal Ube3a in neurons by impeding Ube3a-ATS transcription
a Timeline of behavioral assays performed following ICV injection of AAV-dNmCas9/Nmg15 or AAV-dNmCas9/NmhsaNT27 negative control. b–i Male and female mice are represented by blue and red points, respectively. All boxplot whiskers extend to the highest and lowest values and the middle line indicates the median. Unless otherwise indicated, n = 12–20 mice per group and assays were analyzed by One-way ANOVA with Tukey’s multiple comparisons tests. b, c Hind limb clasping assay (b) total time spent clasping and (c) clasping severity score. Clasp score was analyzed by Kruskal-Wallis test with Dunn’s multiple comparisons tests. d Latency to fall on the accelerating rotarod during trials 1–3 on day 1 and trials 4 and 5 run 48 h later. Error bars indicate SEM. Two-way ANOVA with Tukey’s multiple comparisons tests. e Total distance traveled in the open field during 30 min trial. f Number of marbles buried following 30 min marble burying trial. g Postmortem analysis of brain weight following dissection. h, i Body weights of treated (h) male and (i) female mice. n = 5–15 mice per group. Error bars indicate SEM. Two-way ANOVA with Tukey’s multiple comparisons tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
