Fig. 5: Neonatal injection of AAV-dNmCas9/Nmg15 more potently disrupts Ube3a-ATS and upregulates patUbe3a in cortex than in hippocampus and cerebellum. | Communications Biology

Fig. 5: Neonatal injection of AAV-dNmCas9/Nmg15 more potently disrupts Ube3a-ATS and upregulates patUbe3a in cortex than in hippocampus and cerebellum.

From: AAV-dCas9 vector unsilences paternal Ube3a in neurons by impeding Ube3a-ATS transcription

Fig. 5

a–i RT-qPCR to quantify (a–c) Ube3a-ATS, (d–f) Ube3a, and (g–i) Snord115 expression levels in (a, d, g) cortical, (b, e, h) hippocampal, and (c, f, i) cerebellar samples from WT and AS mice injected with AAV-dNmCas9/Nmg15 or AAV-dNmCas9/NmhsaNT27. Normalized to Eif4a2 and WT control group. Male and female mice are represented by blue and red points, respectively. All boxplot whiskers extend to the highest and lowest values and the middle line indicates the median. n = 12–20 mice per group. One-way ANOVA with Tukey’s multiple comparisons tests, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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