Fig. 7: dNmCas9 exhibits dose-dependent effects on Ube3a-ATS, Ube3a, and Snord115 in vivo.
From: AAV-dCas9 vector unsilences paternal Ube3a in neurons by impeding Ube3a-ATS transcription
a–i Correlation of (a–c) Ube3a-ATS, (d–f) Ube3a, and (g–i) Snord115 expression levels in (a, d, g) cortical, b, e, h hippocampal, and (c, f, i) cerebellar samples from WT and AS mice injected with AAV-dNmCas9/Nmg15 or AAV-dNmCas9/NmhsaNT27 relative to dNmCas9 expression. Relative dNmCas9 expression was normalized to Eif4a2. Ube3a-ATS, Ube3a, and Snord115 expression were normalized to Eif4a2 and WT control group. n = 12-20 mice per group. Lines of best fit (linear regression) are included for all groups, and goodness of fit (r2) and p are indicated for groups exhibiting a significant correlation. Pearson’s r correlation, two-tailed, p < 0.05.
