Fig. 3: C/EBPβ regulates ovarian reserve establishment via p300.

A Detection of the enrichment level of the Ep300 promoter fragment bound to C/EBPβ using CUT&RUN-qPCR. B Analysis of mRNA expression levels of Ep300 in the Ctrl and siCebpb groups. C Representative images (left) and expression level analysis (right) of p300 in the Ctrl and siCebpb groups. D Representative images (left) and line scan analysis (right) of the cellular localization of p300 in the Ctrl and siCebpb groups. White dashed lines indicate the approximate outlines of the nests; yellow arrow indicates the direction of the line scan. E Fluorescence intensity analysis of the nuclei and cytoplasm of germ cells in the nests and follicles in the Ctrl and siCebpb groups. F Representative images (left) and expression level analysis (right) of p300 protein in the E16.5, PD0, PD3 ovaries. G Representative images and expression level analysis of p300 in the Ctrl and siEp300 groups. H IF staining of DDX4 in p300 knockdown model. DDX4 (yellow) indicates oocytes. Hoechst (blue) indicates nuclei (Nuc). White arrow indicates the direction from the medulla (m) to the cortex (c). Violin plots showing the percentage of oocytes in follicles (I) and the total number of oocytes (J) in each ovarian section of each group. K Analysis of mRNA expression levels of Nobox, Lhx8, and Sohlh1 in the Ctrl and siEp300 groups. L Representative images (left) and expression level analysis (right) of DDX4, LHX8, SOHLH1, and NOBOX proteins in the Ctrl and siEp300 groups. Data are means ± SEM. N = 3–6 indicated the biological repeats, as shown on graphs; n = 5 for the biological repeats of cellular localization analysis experiment.