Fig. 4: p300 regulate NTRK signaling through chromatin accessibility during ovarian reserve establishment.

A The distribution of genomic peaks in the Ctrl and siEp300 groups. B Column graph of DARs between Ctrl and siEp300 groups. C Genomic distribution of downregulated DARs in the siEp300 vs. Ctrl groups. D Venn diagram of the gene set with down-regulated DARs in the promoter region, the DEG set up-regulated at PD0, and the DEG set up-regulated at PD3. E Metascape enrichment analysis of candidate gene sets (Gene set acquisition has been described in the text). F Dot plot shows the expression of NTRK signaling-related genes in metascape enrichment analysis in E16.5, PD0, and PD3 oocytes. G The normalized reads of NTRK core-related genes Furin, Pcsk6, and Rap1a shown by ATAC-seq. H Analysis of mRNA expression levels of Furin, Pcsk6, and Rap1a in the Ctrl and siEp300 groups. I Ranking of TFs binding to the Furin promoter region obtained using The Signaling Pathways Project by binding score. J Detection of the binding activity of C/EBPβ to the Furin promoter region in the Ctrl and siEp300 groups using CUT&RUN-qPCR. K Detection of the enrichment level of Furin promoter fragment bound to p300 using CUT&RUN-qPCR. L Representative images (left) and expression level analysis (right) of FURIN, DDX4, LHX8, and SOHLH1 proteins in the Ctrl and siFurin groups. Violin plots showing the percentage of oocytes in follicles (M) and the total number of oocytes (N) in each ovarian section of each group. Data are means ± SEM. N ≥ 2 per group for the biological repeats of ATAC-seq; n = 5 to 6 for the biological repeats of CUT&RUN-qPCR, qPCR, WB, and oocyte count experiments, as shown on graphs.