Fig. 5: Reproductive phenotypes in p300 inhibition and neurotrophin receptor activation mouse models.
From: CCAAT/enhancer binding protein beta (C/EBPβ) regulates the formation of the ovarian reserve
A Schematic diagram of the mouse model of p300 inhibition and neurotrophin receptor activation. This figure was originally created by the authors using Adobe Illustrator. B Analysis of Furin mRNA levels in ovarian tissues of newborn mice treated with different concentrations of curcumin. C Analysis of Fshr mRNA levels in ovarian tissues of newborn mice treated with different concentrations of B-355252. D NGF levels in the serum of postpartum female mice in the Ctrl, Curcumin, Curcumin+B-355252, and B-355252 groups. E Representative images (left) and expression level analysis (right) of DDX4, LHX8, and SOHLH1 proteins in the Ctrl, Curcumin, Curcumin+B-355252, and B-355252 groups. F IF staining of DDX4 in the Ctrl, Curcumin, Curcumin+B-355252, and B-355252 groups. DDX4 (yellow) indicates oocytes. Hoechst (blue) indicates nuclei (Nuc). G, H Violin plots showing the percentage of oocytes in follicles (G) and the total number of oocytes (H) in each ovarian section of each group. Data are means ± SEM. N = 4 to 6 indicated the biological repeats, as shown on graphs.
