Fig. 6: Inhibition of JNK signaling leads to abnormal nuclear translocation of C/EBPβ.
From: CCAAT/enhancer binding protein beta (C/EBPβ) regulates the formation of the ovarian reserve
A Representative images (left) and expression level analysis (right) of p-C/EBPβT188 protein in the E16.5, PD0, PD3 ovaries. B Schematic diagram of the JNK inhibition (JNKi) model construction during in vitro culture of newborn ovarian. This figure was originally created by the authors using Adobe Illustrator. C Representative images of p-C/EBPβT188, C/EBPβ, p300, and FURIN proteins in the Ctrl and JNKi groups. Expression level analysis of p-C/EBPβT188 (D), C/EBPβ (E), p300 (F), and FURIN (G) proteins in the Ctrl and JNKi groups. H IF staining of DDX4 in the Ctrl and JNKi groups. DDX4 (yellow) indicates oocytes. Hoechst (blue) indicates nuclei (Nuc). White arrow indicates the direction from the medulla (m) to the cortex (c). Violin plots showing the percentage of oocytes in follicles (I) and the total number of oocytes (J) in each ovarian section of each group. K Representative images (left) and line scan analysis (right) of the cellular localization of C/EBPβ in the Ctrl and JNKi groups. White dashed lines indicate the approximate outlines of the nests; white arrow indicates the direction from the medulla (m) to the cortex (c); yellow arrow indicates the direction of the line scan. L Fluorescence intensity analysis of the nuclei and cytoplasm of germ cells in the nests and follicles in the Ctrl and JNKi groups. M Proportion of oocytes with C/EBPβ N/C ratio > 1 in the total number of oocytes in the same ovarian section. Data are means ± SEM. N = 4–9 for the biological repeats of WB and oocyte count experiments, as shown on graphs; n = 6 for the biological repeats of cellular localization and fluorescence intensity analysis experiment, a dot in (L) represents a germ cell.
