Fig. 7: Nuclear translocation of C/EBPβ coordinated by JNK signaling and Lamin B1.
From: CCAAT/enhancer binding protein beta (C/EBPβ) regulates the formation of the ovarian reserve
A Representative images (left) and line scan analysis (right) of colocalization of C/EBPβ and Lamin B1 in the Ctrl and JNKi groups. Lamin B1 (blue) indicates nuclear lamina; dashed white arrow indicates the direction of the line scan. B Schematic diagram of the effect of SP600125 treatment on the nuclear translocation of C/EBPβ in oocytes. C Co-IP showed the interaction between C/EBPβ and Lamin B1 in the Ctrl and JNKi groups. Whole ovarian lysate was used as input. D Summary of C/EBPβ regulation during ovarian reserve establishment. Coordinated control of C/EBPβ transcriptional activity by temporal and spatial effects (a); chromatin accessibility dynamics mediated by C/EBPβ downstream molecule p300 participates in regulating the NTRK signaling required for ovarian reserve establishment (b); terminal effects of C/EBPβ regulation during ovarian reserve establishment (c). Data are means ± SEM. N = 5 for the biological repeats of cellular localization analysis experiment; n = 4 for the biological repeats of Co-IP experiment.
