Fig. 1: Alox5 was closely associated with renal tubular injury in IRI-induced AKI in a mouse model. | Communications Biology

Fig. 1: Alox5 was closely associated with renal tubular injury in IRI-induced AKI in a mouse model.

From: Targeting ALOX5 ameliorates renal tubular injury in ischemia–reperfusion-induced acute kidney injury via inhibition of ferroptosis

Fig. 1

A The schematic graph showing the experimental process. B The heatmap showing the DEGs between the control and IRI-induced AKI groups in the chipset “GSE192532,” which were also ferroptosis-related genes in the FerrDb database. C KEGG analysis showing the enrichment of the above DEGs in the ferroptosis pathway. D H&E and PAS staining showing the renal tubular injury in the IRI–AKI mouse model. E The IHC staining of Alox5 and the immunofluorescence (IF) staining to assess the up-regulated expression of Alox5 following IRI treatment. F Representative EM images of morphological changes in mitochondria cristae shape associated with ferroptosis (red arrow head) in renal tubular cells. Abbreviations: IHC immunohistochemistry, IF immunofluorescence, I/R ischemia-reperfusion, LM light microscopy, EM electron microscopy.

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