Fig. 4: ALOX5 knockdown inhibited hypoxia/reoxygenation-induced ferroptosis in HK2 cells. | Communications Biology

Fig. 4: ALOX5 knockdown inhibited hypoxia/reoxygenation-induced ferroptosis in HK2 cells.

From: Targeting ALOX5 ameliorates renal tubular injury in ischemia–reperfusion-induced acute kidney injury via inhibition of ferroptosis

Fig. 4

A The schematic graph showing the experimental process. B Confocal microscopy results showing lipid peroxides detected by Liperfluo staining after ALOX5 knockdown or overexpression in HK2 receiving H/R treatment. C–F Western blot results showing the relative protein levels of GPX4 and xCT after ALOX5 knockdown in HK2 receiving H/R treatment. n = 3 independent experiments. G–J Western blot results showing the relative protein levels of GPX4 and xCT after ALOX5 overexpression in HK2 receiving H/R treatment. n = 3 independent experiments. Densitometric quantification normalized to β-actin is shown to the right of the immunoblots. All data are presented as mean ± standards errors. *p < 0.05. **p < 0.01. Abbreviations: CTRL control, H/R hypoxia/reoxygenation, NC negative control, oe overexpression.

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