Fig. 5: The interaction between ALOX5 and benzbromarone.

A The schematic graph of the kinetic binding study using biolayer interferometry. B Molecular interaction experiment showing the combination between ALOX5 recombinant protein and BBR in different concentrations. C The steady-state analysis showing the degree of fitting of the molecular interaction curve (KD = 8.1E−05 ± 1.1E−05, R2 = 0.9945). D Molecular docking results showing the combination between BBR and ALOX5. E The 2D image of the potential binding sites of ALOX5 to BBR. F The 3D image of the potential binding sites of ALOX5 to BBR. Abbreviations: BBR benzbromarone.