Fig. 6: Benzbromarone protects renal tubular cells from IRI-induced injury in mice. | Communications Biology

Fig. 6: Benzbromarone protects renal tubular cells from IRI-induced injury in mice.

From: Targeting ALOX5 ameliorates renal tubular injury in ischemia–reperfusion-induced acute kidney injury via inhibition of ferroptosis

Fig. 6

A The schematic graph showing the experimental process. B, C Serum creatinine and blood urea nitrogen levels in the I/R mice model receiving BBR treatment. n = 6 animals. D The pathological examinations of kidney tissues, including the H&E and PAS staining showing the renal tubular injury and the IHC staining to detect the expression of ALOX5. E Representative EM images of morphological changes in mitochondria cristae shape associated with ferroptosis (red arrow head) in renal tubular cells. F–H Western blot results showing the relative protein levels of GPX4 and xCT after ALOX5 knockdown in the kidney tissue of I/R mice model receiving BBR treatment. n = 3 independent experiments. Densitometric quantification normalized to β-actin is shown to the right of the immunoblots. All data are presented as mean ± standards errors. *p < 0.05, **p < 0.01. Abbreviations: BBR benzbromarone, DEX dexamethasone, EM electron microscopy, I/R ischemia–reperfusion.

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