Fig. 1: Competence development under strong O2 limitation in GS medium. | Communications Biology

Fig. 1: Competence development under strong O2 limitation in GS medium.

From: ComK2 represses competence development for natural transformation in Staphylococcus aureus grown under strong oxygen limitation

Fig. 1

a A wild-type strain (St29) expressing GFP under the control of PcomG was grown in a GS medium for 28 h. The oxygen concentration, the percentage of GFP-expressing cells, and OD600 nm were measured every 30 min. Results are presented as mean ± SD. Each experiment has been repeated three times (biological replicates). b Transformation efficiencies of wild type (N315ex w/o Phi), sigH (St45), comK1 (St37), comK2 (St38), sa2107 (St39), and nreC (St118) mutant strains using chromosomal DNA (gray bars) (see Material and methods for details). Results are presented as mean ± SD. For each strain, the experiment was repeated at least eight times (biological replicates). Individual experiments are shown as blue circles. Statistical significance was evaluated by two-way ANOVA with Tukey’s posthoc test as follows: ***P < 0.001 and *P < 0.05.

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