Fig. 2: ComK2 inhibits competence and natural transformation under strong O2 limitation.

The percentage of the population expressing GFP under control of PcomG (St29, St51, St40, and St 41) (a) or Pssb (St50, St61, St64, and St67) (b) in a wild-type background or in the absence of sigH, comK1, or comK2 was determined after 26 h of growth in GS medium. Results are presented as mean ± SD. Each experiment has been repeated at least four times (biological replicates). Individual experiments are shown as blue circles. Statistical significance was evaluated by two-way ANOVA with Tukey’s posthoc test as follows: ***P < 0.001 and *P < 0.05.