Fig. 2: Conversion of energy metabolism in the heart from E16.5 to E18.5.
From: Metabolomic landscape of fetal organ development during late gestation in mice
a OPLS-DA analysis of heart at E16.5 (n = 7) and E18.5 (n = 6). b The KEGG pathway enriched by the top 20 differential metabolites in heart from E16.5 (n = 7) to E18.5 (n = 6). The color of the bubble represents the value of −log10 (p-value). c Experimental design of multi-omics analysis (created with Biorender). Targeted metabolomics, stable isotope tracing, and transcriptome sequencing were applied to analyze the conversion process of heart from E16.5 to E18.5. d The abundance of cis-aconitic acid, malic acid, fumaric acid, succinic acid, glucose 1-phosphate, L-lactic acid in heart at E16.5 (n = 3) and E18.5 (n = 3). (*p < 0.05). e Comparison of fractional enrichment of glucose 6-phosphate, citric acid and fumaric acid through isotope tracing (n = 5 per group; *p < 0.05). f Heatmap of energy metabolism-related gene expression in the heart at E16.5 (n = 3) and E18.5 (n = 3). g Metabolic remodeling process in heart from E16.5 to E18.5 (created with Biorender). The metabolites showed in the box. Red represented upregulation in E18.5, and blue represented downregulation. The significance was analyzed by Student’s t-test, error bars represent mean ± SD.
